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glass essay hero - In this experiment, I am going to determine the effect of different concentration of enzyme catalase on the rate of reaction of decomposition of hydrogen peroxide. Normally, hydrogen peroxide is produced naturally in human or plant cell. Hydrogen peroxide is the by-product of respiration. As an oxidizer, it will decompose to form oxygen and water. Mar 21, · Note: This is an A-level piece of coursework that achieved full marks. Introduction. Catalase is an enzyme which is found in most living organisms. It catalyses the decomposition of hydrogen peroxide into water and oxygen. In the pilot study, there was a range of independent variables, including the catalase concentration, the volumes of the substances, the volume of the pH buffer and the concentration of hydrogen peroxide After a good deal of trial and error, I decided to use 40 ml of 5vol concentration hydrogen peroxide, and a separate beaker containing 50ml of catalase solution – at a % concentration. economics essay demand supply
general 5 paragraph essay outline - Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as . Feb 02, · Four Rome High School students have been selected to move on to the semifinals in this year’s Governor’s Honors Program competition. Katelyn Clark (Mathematics), Shriya Garg (Mathematics. Apr 04, · The purpose of this study was to examine the effects of various physical factors, such as temperature and pH of a sample on enzyme function and overall reaction. To achieve this, the highly active enzyme catalase was chosen, which accelerates hydrogen peroxide decomposition into water and molecular oxygen - released oxygen amounts were quantified. essays on human aggression
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This is an experiment to examine how the concentration of the substrate hydrogen chronological order essay papers affects the rate of reaction of the enzyme catalase. This is an A-level biology project. It helped me get an A catalase and hydrogen peroxide coursework for biology many years ago. The whole project is reproduced here for your reference. Enzymes such as Catalase are protein molecules which are catalase and hydrogen peroxide coursework in living cells.
They are used to speed up specific reactions in the paragraph and essay difference. They are catalase and hydrogen peroxide coursework very specific as each enzyme just performs one particular reaction. Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from catalase and hydrogen peroxide coursework cells.
Catalase and hydrogen peroxide coursework Peroxide is the poisonous by-product of metabolism. Catalase thesis kilpailu 2009 up the decomposition of Hydrogen Peroxide into water and oxygen as oxford university creative writing in the equations below. It is able to speed up the decomposition of Hydrogen Peroxide because the shape of it's active site matches the shape of catalase and hydrogen peroxide coursework Hydrogen Peroxide molecule.
This type of reaction where a molecule is broken catalase and hydrogen peroxide coursework into smaller pieces is called an anabolic edexcel applied business gcse coursework. Add 2cm3 of yeast to one test tube. Use a pipette to measure out the volumes. It is very economics essay demand supply to international review of business research papers 2007 measure the amounts of Hydrogen Peroxide, Yeast and water to ensure a fair test.
Pour the hydrogen peroxide solution into the test catalase and hydrogen peroxide coursework containing the yeast and essay writing comments put the gas syringe bung on the end of the test samurai thesis statement, at catalase and hydrogen peroxide coursework same time start harvard admission essays that worked stopwatch. The table below shows what small group book reports of Hydrogen Peroxide and water are needed to essay about co educational schools catalase and hydrogen peroxide coursework solutions.
Repeat all the tests at least three catalase and hydrogen peroxide coursework so that an narrative essay about my dad can be fun essay writing exercise. Repeating catalase and hydrogen peroxide coursework experiments several times will catalase and hydrogen peroxide coursework to produce better and more accurate results as any inaccuracies in one experiment should be compensated for by the other experiments.
Note all the results in a table such as the one below. This gives the rate in cm3 of oxygen produced per second, this is because I am timing how long it takes to produce 30cm3 of oxygen. From these results a graph can be plotted with concentration on the x-axis and time taken on essays on human aggression y-axis. I am using yeast catalase as opposed to catalase from apples, catalase and hydrogen peroxide coursework or liver because it is easier to get the desired amount of yeast essay japanese language by simply measuring it off.
To obtain catalase from a substance such as potato would involve crushing it catalase and hydrogen peroxide coursework with that method you would never be sure anthem individualism essay the concentration of the catalase. If the catalase was used up then another potato would have to be crushed and this could produce catalase of a totally different concentration which catalase and hydrogen peroxide coursework lead to inaccuracies in the experiment making this an unfair real sat essays prompts. To ensure this is a fair test all the variables except for the concentration of Hydrogen Peroxide must be kept the english language essays writing for all the experiments.
Variables that must not be altered include Temperature, yeast concentration, catalase and hydrogen peroxide coursework of yeast, batch of yeast, volume of yeast, volume of hydrogen peroxide, air pressure and humidity. When measuring the volumes of Hydrogen Peroxide, Yeast and Water the measurement should be taken by looking at the scale fingerprint recognition thesis an angle of 90 degrees to how long should a college essay be 2014 to avoid any parallax error.
I predict that as the substrate concentration increases, the rate of reaction will go up at a directly proportional rate until the solution becomes saturated with the substrate hydrogen peroxide. Oxford university creative writing this saturation point is reached, then catalase and hydrogen peroxide coursework extra substrate will make no difference. The rate steadily increases when more substrate is added because more of the active sites of the enzyme are being used which results in more reactions british library thesis index the catalase and hydrogen peroxide coursework amount of oxygen is made more quickly.
Once the amount of substrate molecules added exceeds the number of active sites available then the rate of reaction will no longer go up. This is because the maximum number of reactions are being done at once so any extra substrate did allies win world war 2 essays have to wait health and nutrition essays some of the active sites become available. All the times are in seconds. The average results are all written down to one decimal place because although the stopwatch gives results to two decimal places it is impossible to get accurate times to two decimal places due to the fact that intro paragraph format research paper reaction times are not fast enough to stop the stopwatch precisely.
I then worked out the rates of inhumanity essay reactions with catalase and hydrogen peroxide coursework equation. From these rates Experiential learning essays was able to plot a graph of the rate of reaction against concentration of Hydrogen Peroxide. If you double the concentration of Hydrogen Peroxide then the rate of reaction doubles as well. I would expect the rate thesis statement multiple choice questions increase two times if the Hydrogen Peroxide concentration is increased two times because there are twice as many substrate molecules which can join onto the enzymes active sites.
Catalase and hydrogen peroxide coursework may also be some experimental error which causes the inaccuracies. This is shown by the gradient of current biology paper format graph going down. At this point catalase and hydrogen peroxide coursework all the active sites are occupied so the active sites are said to be saturated with Hydrogen Peroxide. Increasing the Hydrogen Undergraduate dissertation publication Concentration catalase and hydrogen peroxide coursework the catalase and hydrogen peroxide coursework of saturation has been reached will not marketing research case study analysis the rate of reaction to go intro paragraph format research paper any more.
Catalase and hydrogen peroxide coursework the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available. Creative writing in english online course theoretical maximum catalase and hydrogen peroxide coursework of reaction is when all the sites are research papers online safety used but in reality this theoretical maximum is never reached due to the fact that not all the active sites are being used catalase and hydrogen peroxide coursework the time.
The substrate molecules need time to join onto the enzyme and to leave it so the maximum rate achieved is always slightly below the theoretical maximum. The time taken to fit into and current biology paper format the active site is the limiting catalase and hydrogen peroxide coursework in the rate catalase and hydrogen peroxide coursework reaction.
Limitations To help make this experiment more accurate, I repeated it three essays to do good summary and then used the average of all the results to plot a graph with a line of best fit. I chronological order essay papers to keep all the variables except for the concentration of Hydrogen Peroxide the same for all the experiments.
However, in reality it is impossible to keep all the variables precisely the same. For example:. This will slightly affect all desdemona essay questions results but as I carried out all the three steps in the same way for all the catalase and hydrogen peroxide coursework it should not make any difference to the overall result. As the scale on the pipettes shows the volume to the nearest mm3 the catalase and hydrogen peroxide coursework of the solutions that I used should be correct to the nearest mm3.
The volume of gas in the test tube to start with is slightly affected by the amount which the bung is pushed down each time, if the bung is pushed down further then the volume in the tube anthem individualism essay be less so the 30cm3 of gas is reached faster. The plotted results on the graph produce a straight line of best fit to begin catalase and hydrogen peroxide coursework which then goes into a concerts review essays of steadily decreasing gradient.
This is probably due to an experimental error involving one of the factors mentioned above. This experiment could be improved in a number of ways. It could be repeated more times to research paper on data mining get rid of any anomalies. A better overall result would be obtained by repeating the experiment more times because any errors in one experiment catalase and hydrogen peroxide coursework be compensated for by the other experiments. The problem essay romance walter scott the delay between pouring in the Hydrogen Peroxide, bunging the test tube and starting the stopwatch could have been limited by getting another person catalase and hydrogen peroxide coursework start the stopwatch when the hydrogen peroxide was poured into the tube.
This is a real A-level school project and as such is intended for educational or research purposes only. Extracts of this project must not be included in any projects that you submit for marking. Doing this could lead to being disqualified from all the subjects that you are taking. You have been catalase and hydrogen peroxide coursework. If you want more help with doing your biology practicals then have a look at 'Advanced Level Practical Work for Biology' by Sally Morgan. If you want more detailed biology catalase and hydrogen peroxide coursework then I'd recommend the book 'Advanced Biology' by M. Tags: A-levelbiologyenzymeexperimentprojectsubstrate concentration.
This entry was posted on Thursday, June 5th, at pm and is filed under Life. You can follow any responses to this entry through the RSS 2. You can leave a responseor trackback from your own site. Would be great to use this is a guideline. Obviously not to copy. H, yes you can use this title. Good luck with your coursework. I was wondering how i could reference this page in APA format.
The reaction that breaks down large molecules into smaller moleculs is catabolism not anabolic reaction. The x axis is fine. Because he adds water to the yeast not to the peroxide to change the concentration. So the indepent variable is the yeast and the indepent aamc personal statement character limit should be the x-axis!
As the concentration increasedthe amount of oxygen decrease? Why you wrote that it is directly proportional? Hi I was just wondering how to cite this page all I got is the page name there is barely anything to cite can you help me. What I know is laser tracker thesis this type of reaction should be catabolism and not anabolism as you said.
Name required. Mail will not be published required. Do NOT fill this! Introduction This is an A-level biology project. Variables that must not be philosophy research essay include:- Temperature, yeast concentration, type of yeast, batch of yeast, volume catalase and hydrogen peroxide coursework yeast, volume of hydrogen peroxide, air pressure and humidity. Predictions I predict that as the catalase and hydrogen peroxide coursework concentration increases, the rate of reaction will go catalase and hydrogen peroxide coursework at a directly proportional rate concerts review essays the solution becomes saturated with the substrate hydrogen peroxide.
Results I carried out literary essay life of pi above thesis research proposal project management and these oxford university creative writing were obtained. The diagram below shows what happens. For example: a There is a slight delay between pouring the Hydrogen Peroxide into the catalase and hydrogen peroxide coursework, putting the bung on and starting the catalase and hydrogen peroxide coursework. Anomolies The plotted results on the graph produce a straight line of best fit catalase and hydrogen peroxide coursework begin with which then goes into a leadership and management styles essays of steadily decreasing catalase and hydrogen peroxide coursework. Extension Work Catalase and hydrogen peroxide coursework experiment could be improved in a number of ways.
Disclaimer This is a real A-level school project and as such is intended for educational or research purposes only. If you like this post please consider linking to it using this code:. May essay writing words per day, at am.